Establishment of Sequence-Tagged Sites on 15q11-q13 byAlu-Vector PCR Cloning of Yac-Generated Fragments
نویسندگان
چکیده
منابع مشابه
Cloning and analysis of PCR-generated DNA fragments.
Methods are presented for the improved yield and analysis of blunt-ended cloning of PCR-generated DNA fragments. We show that Pfu DNA polymerase polishing of Taq DNA polymerase-generated fragments increases the yield and efficiency of cloning. Using a triple primer set consisting of two outside, asymmetrically distanced primers and one fragment-specific primer, both the presence and orientation...
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ژورنال
عنوان ژورنال: Disease Markers
سال: 1996
ISSN: 0278-0240,1875-8630
DOI: 10.1155/1996/167830